The present study deals with the administration of Bacillus coagulans (LMG S-31876) 50 billion CFU/g (Colony Forming Unit/gram) on mice. The strain has been isolated from fermented rice. A study on acute and sub-acute toxicity of the strain was analyzed on the Wistar rats at the dose levels of 100-400 mg/kg body weight; the maximum dose volume was maintained at 20 ml/kg body weight for a consecutive period of 90 days. The objectives of the study were to assess the toxicological profile of the test item when administered by oral gavage to Wistar rats for a period of 90 consecutive days and assess the potential reversibility of any findings, and thereby provide data to support the use of the test item in humans. This study exhibited no significant clinical signs, body weight, haematology, or clinical chemistry changes before and after treatment as compared to the vehicle control group. Thereby, the test item is safe to be utilized as a probiotic product.