Background: There are many physiotherapy interventions used in treatment of osteoarthritis and among them are, positional release technique and active release technique. Objective: The purpose of this study is to compare and contrast the immediate effectiveness of ART and PRT on hamstring in patients with osteoarthritis knee. Methodology: 30 patients with osteoarthritis knee were divided into two groups based on selection criteria. Group A received PRT and group B received ART on hamstring. NPRS and active knee flexion ROM using goniometry were used as outcome measures. Result: On comparing pre to post intervention values in group A and group B, there was significant improvement noted in terms of pain and knee ROM statistically, in both groups, but in comparison not much difference is seen in between both the techniques. Conclusion: This study concluded that both the groups showed improvement statistically and clinically in terms of pain and knee flexion ROM.