The effects of thymoquinone on small cell lung cancer cell lines
Bayrakçı Onur, Taşdemir Karaman Demet, Işık Ahmet Ferudun
Lung cancer is one of the most common types of cancer that causes death. Small cell lung cancer seen in 14%, is a type of cancer that is closely related to smoking and has an aggressive course. It is a metastatic tumor when diagnosed. In this stage where surgical treatment cannot be performed, the primary treatment is chemotherapy. Cisplatin is amongthe options in chemotherapy combinations. In vivo and in vitro studies have shown the antitumoral and antineoplastic effects of the bioactive component thymoquinone obtained from the Nigella Sativa plant. Therefore, our study aimed to target the effects of thymoquinone and cisplatin on small cell lung cancer cell lines. Small cell lung cancer cell lines were cultured in accordance with international standards. The cells were treated with 100 μM thymoquinone and 200 μM cisplatin for 4h under incubation conditions. We used dimetil sülfoksit (DMSO) for negative control (thymoquinone and cisplatin solved in DMSO). Experiments were repeated 4 times in the same laboratory, by the same person, at different times. In conclusion, the effects of thymoquinone and cisplatin were statistically significant at 10,100,200 μM concentration compared with DMSO separately. The effective concentration of thymoquinone was detected to be at a concentration of 100 μM, and that of cisplatin at a concentration of 200 μM. 100 μM thymoquinone is more effective and statistically significant than both 100 μM and 200 μM cisplatin. 200 mM thymoquinone is more effective than 200 μM cisplatin.
Bayrakçı Onur, Taşdemir Karaman Demet, Işık Ahmet Ferudun. The effects of thymoquinone on small cell lung cancer cell lines. International Journal of Medical Science and Clinical Research, Volume 3, Issue 3, 2021, Pages 31-33